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dhbB [2019-08-22 11:23:37]
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dhbB [2019-08-22 11:23:37]

isochorismatase
locus
BSU_31970
pI
4.43
mw
34.95 kDa
protein length
312 aa Sequence Blast
gene length
939 bp Sequence Blast
function
biosynthesis of the siderophore bacillibactin
product
isochorismatase
essential
no
ec
3.3.2.1
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.3|Acquisition of iron / Other]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.1|Acquisition of iron / Other]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.5|Other proteins required for biofilm formation]

    • Gene

    Coordinates
    3,287,675 3,288,613

    Phenotypes of a mutant

  • defective in biofilm formation [pubmed|31420537]

    • The protein

    Catalyzed reaction/ biological activity

  • Isochorismate + H2O --> 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate (according to UniProt)

    • Protein family

  • [SW|Isochorismatase family] (according to UniProt)

    • Structure

  • [PDB|2FQ1] (from E. coli, 49% identity) [pubmed|16632253]

    • Expression and Regulation

    Operons

    genes
    [gene|42C8D303AABFA2A9706295A0F90B0B49F854B0BF|dhbA]-[gene|0D4D200095AD4F9B30179B9AF500306EDA61F5D1|dhbC]-[gene|B864EC6D21AD5E524AC13AE1BE41F5CD7FC399C5|dhbE]-[gene|1346D57F906EE5BD36F7FBFA1E4884EEBC8585FA|dhbB]-[gene|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|dhbF]-[gene|DD647083C2E6968AD26A41C9F4EFBA942D75207C|ybdZ]
    description
    [Pubmed|8921902]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8550523], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI]: sigma factor, [pubmed|29914988], in [regulon|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI regulon]

    • regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|Kre]: repression, in [regulon|65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|Kre regulon]

    • regulation

  • induced by iron starvation (second wave to allow iron scavenging from the environment) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]) [Pubmed|29133393,12354229]
  • expression of the operon is strongly induced at the beginning of [SW|biofilm formation] [pubmed|31113899]

    • additional information

  • the ''[gene|42C8D303AABFA2A9706295A0F90B0B49F854B0BF|dhbA]-[gene|0D4D200095AD4F9B30179B9AF500306EDA61F5D1|dhbC]-[gene|B864EC6D21AD5E524AC13AE1BE41F5CD7FC399C5|dhbE]-[gene|1346D57F906EE5BD36F7FBFA1E4884EEBC8585FA|dhbB]-[gene|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|dhbF]'' operon is strongly unregulated in a ''[SW|kre]'' mutant [Pubmed|26110430]
    • view in new tab

    Biological materials

    Mutant

  • BKE31970 ([gene|1346D57F906EE5BD36F7FBFA1E4884EEBC8585FA|dhbB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGAACACTTCCTCTCA, downstream forward: _UP4_TTATAAAAGGAGGGTAAACA
  • BKK31970 ([gene|1346D57F906EE5BD36F7FBFA1E4884EEBC8585FA|dhbB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGAACACTTCCTCTCA, downstream forward: _UP4_TTATAAAAGGAGGGTAAACA

    • References

  • 11112781,8921902,11790741,12354229,8550523,20817675,23033921,21815947,26110430,29133393,29914988,16632253,31420537